Workflow: plasmid sequencing

Rapid plasmid analysis with nanopore sequencing and EPI2ME workflows

At the core of a researcher’s ability to synthesise novel proteins is the plasmid construct, containing genes coding for proteins of interest, as well as accessory components offering control of expression or genetic features for selective pressure options. Nanopore sequencing enables the highly accurate, flexible, and secure characterisation of full-length plasmids in-house, with results obtained in hours — negating the need to send constructs to third parties for validation. This end-to-end workflow is a flexible, rapid method for sequencing and assembling plasmid constructs.

In this workflow, you will:

• Find out how full-length plasmid sequencing enhances construct validation
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices