Workflow: tumour-normal sequencing

Characterising genomic and epigenomic variation between tumour-normal research samples using long nanopore sequencing reads

Paired tumour-normal whole-genome sequencing enables a deeper understanding of genomic and epigenomic variability in cancer, which can prompt the discovery of new cancer biomarkers and new insights into the genetics of treatment-resistant tumours. With nanopore sequencing, long, native DNA reads enable the capture of single nucleotide variants (SNVs), structural variants (SVs), copy number variants (CNVs), short tandem repeats (STRs), and epigenetic modifications, including 5mC and 5hmC, in a single dataset. This end-to-end workflow provides a simple solution for detecting a wide range of tumour-specific variation in a single sequencing assay.

In this workflow, you will:

• Find out how native DNA nanopore sequencing enhances cancer research
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices