Workflow: bacterial isolate sequencing

Nanopore-only microbial isolate sequencing solution (NO-MISS): a flexible and rapid approach for whole-genome sequencing of bacterial isolates

Whole-genome sequencing of microbial isolates provides valuable information for public health, clinical microbiology research, food safety, and microbial ecology. Nanopore sequencing can generate reads of unrestricted read length to deliver reference-quality, complete genome assemblies, including repeat-rich regions and mobile genetic elements. This end-to-end workflow provides a rapid solution for whole-genome sequencing of bacterial isolates.

In this workflow, you will:

• Find out how nanopore-only, whole-genome sequencing of isolates generates reference-quality bacterial genome assemblies
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices